Sodium alginate has been widely used as a pharmaceutical excipient. Modern pharmacological studies have shown that it has an auxiliary effect on the prevention and treatment of tumors, hypertension and the accumulation of metal ions such as Cd2+ and Pb2+ in the human body. The degradation product of sodium alginate is Alginate Oligosaccharide, which has the characteristics of easy absorption, strong water solubility and higher biological activity compared with sodium alginate.
Zebrafish embryos were selected as animal experimental models to determine the ability of sodium alginate with different molecular weights to reduce the toxicity of Cd2+ and Pb2+. Different molecular weights of sodium alginate combined with Cd2+ and Pb2+ were used on zebrafish embryos. The results showed that the strongest protective effects of sodium alginate with average molecular weights of 1575 D, 7075D and 61518D on the death of zebrafish embryos caused by cadmium treatment were 5 mg/ L, 10 mg/L and 50 mg/L; only 1575 D sodium alginate has protective effect on Pb2+-induced zebrafish developmental toxicity, the optimal protective concentration is 25 mg/L, 7075 D and 61518 D sodium alginate have no obvious protection effect. Sodium alginate with different molecular weights can reduce the accumulation of Cd2+ in whole eggs and embryos of zebrafish, improve embryonic cell apoptosis caused by Cd2+, reduce the levels of MDA and ROS, and increase the activity of SOD, especially the activity of low molecular weight sodium alginate the strongest. Low molecular weight sodium alginate has similar intervention effect on the developmental toxicity of zebrafish embryos induced by Pb2+.
Experiments show that both Cd2+ and Pb2+ can induce developmental toxicity and oxidative stress damage in zebrafish embryos. After degradation, 1575 D low molecular weight sodium alginate can significantly improve the accumulation of Cd2+ and Pb2+ in zebrafish eggs and embryos, and significantly improve embryonic oxidation. Stress levels, reduce apoptosis, and protect against Cd2+ and Pb2+-induced developmental toxicity in zebrafish embryos. This study provides a simple and quick solution for removing heavy metals from the human body and for clinical research.
The article is from "Protective effects of different molecular weight sodium alginate on the developmental toxicity of zebrafish embryos induced by Cd2+ and Pb2+"
Sales Office