From:DOI: https://doi.org/10.21203/rs.3.rs-3146201/v1
Combined Transcriptome and Metabolome Analysis of Alginate Oligosaccharides Alleviating Salt Stress in the Rice Seedling Stage
Background: Salt stress is one of the main limiting factors of rice production. Alginate oligosaccharides can enhance plant stress resistance. However, the molecular mechanism of the benefits of alginate oligosaccharides on rice salt tolerance remains unclear. Rice FL478 and IR29 were used to comprehensively analyze the effects of leaf spraying alginate oligosaccharides on the transcriptomes and metabolites of rice seedlings under salt stress.
Results: In this experiment, exogenous application of alginate oligosaccharides reduced the leaf membrane damage, increased the aboveground and below rice biomass, and improved rice salt tolerance. Comparative transcriptomic analyses showed that alginate oligosaccharides regulated the genes related to light-trapping proteins and RLCK receptor cytoplasmic kinases, including CBA, LHCB and Lhcp, in response to salt stress. In the metabolome profile study, among 22 and 50 different metabolites treated with alginate oligosaccharides + salt in FL478 and IR29, the abundance of metabolites with antioxidant activity, such as 6-Hydroxymelatonin, Wedelolactone and L-Histidine increased significantly.Combined transcriptome and metabolome analyses revealed that dehydroascorbic acid in the glutathione and ascorbic acid cycles plays a vital role in salt tolerance mediated by alginate oligosaccharides.
Conclusion: Alginate oligosaccharides activate multiple pathways to cope with the adverse effects of salt stress. This study provides a molecular basis for alginate oligosaccharides alleviating salt stress damage in rice.