The types of soil microbial populations have an important impact on soil quality and crop growth, and the characteristics of the effects of oligosaccharides on soil microbial populations were studied. , which contributes to the correct, efficient and safe use of oligosaccharides. The experiment was carried out in an artificial climate chamber for soil cultivation, 50 mg·L-1 of chitosan oligosaccharide solution was applied to the soil, and clear water (CK) was used as the control treatment. After 6 days of cultivation, samples were taken. High-throughput sequencing technology was used to analyze the structure and diversity of soil biological communities. Sex distribution characteristics.
Compared with the CK group treated with chitosan oligosaccharide group, Proteobacteria (26.96%-31.03%), Acidobacteria (14.85%-22.97%), Actinobacteria (14.28%-16.71%), Chloroflexi (14.28%-16.71%) 7.13%~8.20%) and Bacteroidetes (6.84%~7.27%), accounting for more than 86% of the total colonies. After chitosan oligosaccharide treatment, Proteobacteria, Actinomycetes and Bacillus phylum increased by 15%, 17%, and 19%, respectively. The soil fungal community structure was significantly improved after oligosaccharide treatment.
This study found that chitosan oligosaccharides had the highest influence on the abundance of Proteobacteria, Actinobacteria and Acidobacteria, which contributed to the accumulation of soil nutrients. Actinomycetes play an important role in soil carbon and nitrogen cycling, and can also produce spores with strong resistance to stress. At the same time, they have functions such as dissolving phosphorus and potassium. With the increase of carbon content in the soil environment, it is also conducive to bud formation. The abundance of Bacterial phyla increased.
The results showed that chitosan oligosaccharide treatment could significantly increase the abundance of MND1. Lysobacter, Nitrospira, Actinomyces, Bacillus, Halobacterium ochracea, Bacillus and Streptomyces. . As a phosphate-solubilizing microorganism, Aspergillus flavus can effectively improve the supply of soil available phosphorus, and the relative abundance of chitosan oligosaccharide can be increased by 195% after use.
Chitooligosaccharide treatment can promote the growth of a variety of beneficial bacteria that are beneficial to soil nutrients.
From Qian Yuanchao et al., "The Regulation of Oligosaccharides on Soil Microbial Diversity and Community Structure"
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