Chitosan oligosaccharide is a low molecular weight water-soluble glucosamine degraded by chitosan. it consists of 2~20 monosaccharide units and has the advantages of good water solubility and degradability. Chitosan oligosaccharides have many pharmacological activities, such as anti-inflammatory, bacteriostatic, enhancing immunity and regulating blood lipids. This study investigated the effect of chitosan oligosaccharides on blood glucose in rats with tetracil diabetes.
A rat model of diabetes was established by tail vein injection of alloxan, which was randomly divided into model control group, high, medium and low dose groups of chitosan oligosaccharides (600,300,150 mg/kg of chitosan), metformin group and normal control group (150 mg/kg of metformin were given by gavage),6 in each group. another 6 unmodeled rats were taken as the normal control group, and the normal control group and the model control group were given equal volume of distilled water. To observe the effect of chitosan oligosaccharide on blood glucose and insulin in diabetic rats, and to observe the effect of chitosan oligosaccharide on blood glucose and glucose tolerance in normal mice.
Results, the blood glucose in the model group was significantly higher than that in the normal control group. After administration, the blood glucose in the lower, middle and high dose groups and metformin groups decreased by 43.3%,39.8%,44.5%,53.0%, respectively. After administration, the insulin in the lower, middle and high dose groups and metformin groups increased by 9.0%,11.4%,41.0%,12.7%, respectively. effects of chitosan oligosaccharides on blood glucose in normal mice and oral glucose tolerance in mice showed that there was no significant difference between the blood glucose in mice with low, medium and high doses of chitosan and the blood glucose h 0,1 and 2 in mice with blank control group. chitosan oligosaccharides reduced blood glucose 0.5 h after gastric glucose administration in mice. after administration of glucose 0.5 h, the blood glucose in mice with low, medium and high dose groups decreased 12.2%,31.8% and 25.2% respectively compared with the control group.
This study shows that chitosan oligosaccharides have obvious hypoglycemic effect, can enhance the load glucose tolerance of mice, improve the function of islet cells, and have the trend of increasing insulin in high blood. The effect of chitosan oligosaccharide on lowering blood glucose may be related to its action on insulin receptor, which makes insulin receptor sensitivity increase, thus controlling the increase of blood glucose.