Immunomodulatory effect of mannuronic oligosaccharides

Mannouronic acid oligosaccharides are made by enzymatic hydrolysis of alginate M. It has the functions of anti-senile dementia, improving immunity, anti-tumor, anti-oxidation, lowering blood sugar and blood lipids. In order to study the effect of mannuronic acid oligosaccharides on the immune function of mice, the experiment used the MTT method to determine the effect of mannuronic acid oligosaccharides on the proliferation of spleen cells, and the effects of concanavalin A (Con A) and lipopolysaccharide (LPS) were tested. The effect of spleen lymphocyte proliferation caused by mice; neutral red method was used to determine the effect of mannuronic acid oligosaccharides on the phagocytic activity of macrophages; the serum hemolysin method was used to determine the effect of mannuronic acid oligosaccharides on chicken red blood cells The effect of mouse hemolysin antibody production. Results Mannouronic acid oligosaccharides can promote the proliferation of mouse spleen cells and increase mouse spleen index. At the same time, mannuronic acid oligosaccharides can promote the proliferation of T lymphocytes and B lymphocytes induced by ConA and LPS, indicating that mannuronic acid can improve the body's cellular and humoral immunity. Mannouronic acid oligosaccharides can obviously promote the ability of peritoneal macrophages to swallow neutral red, suggesting that mannuronic acid oligosaccharides can improve the body's non-specific immunity. Mannuronic acid can also significantly increase the content of serum hemolysin in mice. In summary, mannuronic acid oligosaccharides can improve the body's humoral immunity, cellular immunity and non-specific immunity. This effect may be one of the mechanisms by which it increases white blood cells in mice.