Dosage
Foliar spray induction: 1500 times of 5% chitosan oligosaccharide solution was prepared and sprayed every 3 days, 3 times in total.
Root irrigation induction: 1000 times of 5% chitosan oligosaccharide solution was prepared, and the roots were irrigated once every 3 days, 3 times in total.
Experimental effect:
5% Chitooligosaccharide Foliar Spray and Root Irrigation Treatment to Improve the Resistance of Bitter Gourd Seedlings to Fusarium Wilt: Comparison of the Best Induction Effects of Two Fusarium Wilt Resistance Induction Methods The induced disease resistance index (70.39) of seedling resistance to Fusarium wilt was 27.30 higher than that of 5% chitosan oligosaccharide 1500 times liquid spray (40.09). Chitosan oligosaccharide root irrigation was better than spraying in improving the induction and resistance of bitter gourd.
The induction of 5% chitosan oligosaccharide can delay the infection time of Fusarium wilt, prevent the further development of hyphae and inhibit the formation of spores.
Different degrees of lignin deposition were found in the roots, stems, leaves and other tissues of bitter gourd induced by 5% chitosan oligosaccharide.
After induction with 5% chitosan oligosaccharide, different degrees of phenolic compounds were deposited in the roots, stems and leaves of bitter gourd seedlings.
Summary:
Chitosan oligosaccharide, as a new type of plant immune inducer, has a good effect on the induction of disease resistance, and has attracted more and more researchers' attention. Studies have shown that chitosan oligosaccharide can induce resistance to verticillium wilt in cotton, resistance to early blight in tomato, resistance to brown edge gray spot in banana, resistance to downy mildew in grape, resistance to Fusarium wilt of bitter gourd and resistance to canker of dragon fruit.
The induction of 5% chitosan oligosaccharide can stimulate the initiation of the disease-resistant immune system of bitter gourd, and increase the PPO (polyphenol oxidase), PAL (phenylalanine ammonia lyase), POD (peroxidase) and β-1 in the roots of bitter gourd seedlings ,3-GLU (β-1,3-glucanase) activity, promotes the deposition of lignin and phenolic substances in the cell wall of the root, stem and leaf tissue of bitter gourd, increases the degree of lignification, and enhances the defense ability of bitter gourd against fusarium wilt, It can delay the expansion speed of Fusarium wilt of bitter gourd and effectively induce the resistance of Fusarium wilt of bitter gourd.
The article is derived from "resistance induction of aminooligosaccharides to Fusarium wilt of bitter gourd"
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