As we all know, Oxidative stress(OS) is the major threat to human health, which can lead to damage to the reproductive system, cardiovascular disease, and other diseases. Despite remarkable progress in understanding the endogenously produced antioxidants in semen, exploration of exogenous antioxidants with better efficacy and no side effect attracts a great deal of attention in the field of male infertility.
Chitosan and its derivatives exhibit great antioxidant potential in various diseases，thereby exerting the effect of anti-inflammation, immunostimulation, anti-tumor, and promoting tissue regeneration . Here, we show that chitosan oligosaccharide (COS), a degradation product of chitosan with a degree of polymerization of less than 10, is a strong antioxidant in spermatogonia cells. COS treatment reduces t-BHP-induced reactive oxygen species overproduction and programmed necrosis in GC-1 spg cells, and improves mitochondrial functions and cell viability by inhibiting excessive endoplasmic reticulum stress. This findings provide novel insight into the protective role of COS in germ cell damage.
(a) DCFH-DA was used to detect intracellular ROS. Scale, 200 μm. Green fluorescence represents DCFH-DA, which also represents ROS level. (b) Cell quantification was observed by green fluorescence. The results were
presented as x ± SD (n = 3). ***p < .001 compared with the t-BHP group, ###p < .001 compared with the control group, ns indicates not statistically significant.
Reference:Chitosan oligosaccharides attenuate programmed necrosis induced by oxidative stress in spermatogonia cells
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